Thus, receptor overexpression, together with a similar expression in both the primary tumors and the disseminated lesions, is considered necessary for the success of targeted nuclide radiotherapy. EGFR is overexpressed in up to 80% of NSCLC [16–18]. However, it is still uncertain whether the EGFR protein expression determined in the primary tumors exactly reflects the EGFR status of the metastatic tumors in NSCLC patients. In the present study, the EGFR expression was investigated
immunohistochemically in a series of 51 primary NSCLC samples and corresponding lymph node metastases. The goal was to evaluate whether the receptor is suitable as target for clinical therapy, including radionuclide based therapy. Methods selleck products Patients and Samples Patients with NSCLC who were treated with curative resection for excision of primary tumor and corresponding lymph nodes metastases, between 2006 and 2007, were enrolled in the present study. Tumor samples from all patients were obtained at the time of operation through the Thoracic Surgery (Oncology) Department and the Pathology Department, Ningbo Second Hospital, under approval of the Institutional Review Board in accordance with the Declaration of Helsinki. Paraffin sections from both the primary tumors and the corresponding lymph node metastases were required for inclusion. Tissue samples were not taken from distant metastases so these were not available for analysis.
Patients who had received preoperative thoracic radiotherapy or preoperative A-1155463 solubility dmso systemic chemotherapy were excluded. Patients who had received anti-EGFR therapy were also excluded. Totally, selleck chemicals 51 patients were finally included in the study. Clinical information was obtained from the hospital records and included patient age, gender, disease stage, and histological pattern. Lung cancer histology was defined according to the World Health Organization pathology classification . Clinicalpathologic staging was determined according to the International Union Against Cancer tumor-node-metastasis
classification of malignant tumors . The patient and tumor characteristics of the analyzed cases are shown in Table 1. Table 1 Tumour and patient characteristics (n = 51) Characteristics Patients, n (%) Histamine H2 receptor Age at diagnosis, years Medium 61 Range 40-78 Gender Male 35 (68.6) Female 16 (31.4) Histology Squamous cell carcinomas 18 (35.3) Adenocarcinomas 27 (52.9) Bronchioloalveolar carcinoma 2 (3.9) Adenosquamous carcinoma 4 (7.8) T-stages of the primary lesions T1 8 (15.7) T2 32 (62.7) T3 5 (9.8) T4 6 (11.8) N-stages N1 20 (39.2) N2 28 (54.9) N3 3 (5.9) M-stages M0 46 (90.2) M1 5 (9.8) Stages at diagnosis II 13 (25.5) IIIA 29 (56.9) IIIB 4 (7.8) IV 5 (9.8) EGFR-staining The tissues were fixed in 4% buffered formalin, processed and embedded in paraffin.