Materials and methods: Hscen 2 was submitted to hydroxyl and azide radicals produced by radiolysis in the absence of oxygen. The resulting products were characterised by biochemical, spectroscopic and mass spectrometry techniques. Their thermodynamics parameters of complexation with C-terminal fragment of Xeroderma pigmentosum C protein (C-XPC), one of the Hscen 2 cellular partners, were quantified by isothermal titration calorimetry (ITC).
Results: Both hydroxyl and azide radicals induce centrin 2 polymerisation as we characterised several
intermolecular cross-links generating dimers, trimers, tetramers and higher molecular mass species. These cross-links result from the formation of a covalent bond between the only tyrosine residue (Tyr 172) located in the C-terminal region of each monomer. Remarkably, dimerisation occurs for doses as low as a few grays. Moreover, this Hscen2 dimer has a lower affinity BTSA1 and stoechiometry binding to C-XPC.
Conclusions: These results show that as oxidative radicals induce high proportions of irreversible damages (polymerisation) centrin 2 is highly sensitive Navitoclax to ionising radiation. This could have important consequences on its biological functions.”
“Structural and magnetic properties
of epitaxial Co2FeAl Heusler alloy thin films were investigated. Films were deposited on single crystal MgO (001) substrates at room temperature, followed by an annealing process at
600 degrees C. MgO and Cr buffer layers were introduced in order to enhance crystalline quality, and improve magnetic properties. Structural analyses indicate that samples have grown in the B2 ordered epitaxial structure. VSM measures show that the MgO buffered sample displays a magnetization saturation of 1010 +/- 30 emu/cm(3), and Cr buffered sample displays a magnetization saturation of 1032 +/- 40 emu/cm(3). Damping factor was studied by strip-line ferromagnetic resonance measures. We observed a maximum value for the MgO buffered sample of about 8.5 x 10(-3), and a minimum value Selleck SHP099 of 3.8 x 10(-3) for the Cr buffered one. (C) 2011 American Institute of Physics. [doi:10.1063/1.3549581]“
“Background: Studies have shown that the presence of left atrial (LA) fibrosis can be assessed by LA delayed-enhancement cardiac magnetic resonance (LA DE-CMR) and may be predictive of outcome after ablation for atrial fibrillation (AF). We sought to test the hypothesis that the amount of LA fibrosis evaluated by DE-CMR correlates with the difficulty of complex fractionated atrial electrograms (CFAE) ablation.
Methods: Twenty-two consecutive patients (86.4% nonparoxysmal AF) underwent substrate CFAE radiofrequency (RF) ablation (+/- Pulmonary veins isolation) with AF termination as the endpoint. LA DE-CMR was performed prior to ablation.