Here, we review recent mass spectrometry-based studies of Alzheimer’s disease CSF, and additionally discuss issues associated with CSF in proteomics studies.”
“Background: A sedated, mature male miniature swine hemorrhage model has been specifically developed to evaluate resuscitation
products for the Defense Advanced Research Projects Agency Surviving Blood Loss program.
Methods: Animals were placed in a sling, sedated with midazolam, and hemorrhaged 60% of estimated blood volume (similar to 39 mL/kg) exponentially for 1 hour with no resuscitation (control; n = 16). An additional 26 swine were treated similarly, then resuscitated with 1 mL/kg/min of Hextend to a systolic blood pressure of either 65 mm Hg +/- 2 mm Hg (n = 7) or 80 mm Hg +/- 5 mm Hg (n = 7) and with 17 beta-estradiol (E2) at 1 mg/kg (n = 6) or 10 mg/kg (n = 6). Animals were observed for 3 hours with periodic blood sampling. Survival times for the two E2 groups see more were not significantly different (p = 0.59); therefore, the groups were combined for
comparison with control.
Results: Hemorrhage resulted in a characteristic hypotension and metabolic acidosis. Survival time for the control swine was 64 minutes +/- 11.5 minutes with a 6% survival at 180 minutes. The 180 minutes Hextend survival was 86% for 65 mm Hg and 100% for 80 mm Hg. E2 survival was 125 minutes +/- 15.3 minutes, significantly different from control (p = 0.01), but E2 survival of 25% at 180 minutes was not different from control.
Conclusion: A sedated, sexually mature male miniature swine severe hemorrhage model has been successfully developed, LY2090314 in vivo resuscitated with Hextend and used to evaluate E2 as a small volume resuscitation product.”
“Objective.
Benign and malignant tumor cells can express altered adhesion properties, and these features can be associated with their proliferative and invasive characteristics. This study aimed to evaluate syndecan-1 Nutlin-3 cost and Ki-67 expression in ghost cell-containing odontogenic tumors.
Study Design. Clinical data were retrieved from laboratory records, and hematoxylin-eosin-stained slides and sections, labeled with monoclonal antibodies anti-syndecan-1 and anti-Ki-67 using the immunoperoxidase technique, were evaluated.
Results. Included were 21 central calcifying cystic odontogenic tumors (CCOTs) (4 associated with odontoma), 2 peripheral CCOTs, 1 dentinogenic ghost cell tumor, and 1 ghost cell odontogenic carcinoma (GCOC). Syndecan-1 was mainly expressed in cells resembling stellate reticulum and in stromal cells from the fibrous capsule. The mean Ki-67 labeling index was 4.1% (49.3% for GCOC), but it was not associated with syndecan-1 expression.
Conclusions. Syndecan-1 is variably expressed in cells resembling the stellate reticulum, stromal cells, and basal cells and might be associated with the biology of these tumors.