65) and the adjusted
R2 up slightly (to 0.367) (Additional file 3: Table S1). Variable selection to achieve a model of rosetting In order to identify what genetic variation best explains the variation observed in rosetting, we performed a variable selection procedure to find the optimal set of MLN2238 independent variables for a multiple regression model of rosetting. Three tests were performed, which together show that HB 219 is a better predictor of rosetting than any of the classic var types (Table 1): Table 1 Statistics for multiple regression models predicting rosetting* Independent variables AIC BIC R2 Adj. R2 A Cys2, Grp2, Grp3, BS1CP6 20.14 37.40 0.358 0.338 B HB36, HB204, HB210, HB219, HB486 16.48 BI 2536 price 36.60 0.385 0.361 C BS1CP6, HB54, HB171, HB204, HB219 14.02 34.14 0.400 0.373 D BS1CP6, PC1, PC3, PC4, PC22 4.776 24.90 0.438 0.415 *The result of removing the least
significant genetic variable, one by one, from models of rosetting that start with the expression rates of: (row A) the 7 classic var types, (row B) the 29 HB expression rates, (row C) the expression rates for both EX 527 the 7 classic var types and the 29 HBs, and (row D) the expression rates for the 7 classic var types and the 29 PCs. The variable selection procedure is done maintaining host age in the model, however statistics are shown with age removed. Positive effect independent variables are shown in boldface. In a first test, we start with a model that initially includes all seven classic var types plus host age. We successively remove the genetic variable that contributes least significantly to the model until the BIC and related statistics are optimized (see Methods for details). We find that the model with the lowest BIC contains the expression rates for cys2 and BS1/CP6 var types as positive predictors of rosetting, and the expression rates for cysPoLV group 2 and cysPoLV group Interleukin-2 receptor 3 var types as negative predictors of rosetting (BIC = 37.40) (row A in Table 1 and Additional file
3: Table S3). In a second test we start with all 29 HB expression rates plus host age as independent variables and then we follow the same variable selection procedure. In this case the resulting model is one with HB 36, HB 204 and HB 210 as negative predictors of rosetting, and HB 219 and HB 486 as positive predictors of rosetting (BIC = 36.60) (row B in Table 1 and Additional file 3: Table S3). In a third variable selection test we start with all 29 HB expression rates in addition to the expression rates for all seven classic var types, plus host age. Starting with this initial set of independent variables, the model that results after variable selection is one containing the expression rates of BS1/CP6 and HB 219 as positive predictors of rosetting, and the expression rates of HB 54, HB 171 and HB 204 as negative predictors of rosetting (BIC = 34.