We observed that regular administration of ABT 510 until euthanasia completely inhibited the development of human malignant astrocytoma tumors established inside the brains of athymic nude mice. The microvessel density was drastically reduced and also the amount of apoptotic MvECs was appreciably greater during the tumors of ABT 510 taken care of animals. Comparable final results have been found through which an intracerebral malignant glioma propagated within a syngeneic mouse model. ABT 510 treat ment of major human selelck kinase inhibitor brain MvECs propagated like a monolayer resulted in induction of apoptosis in a dose and time dependent method through a caspase eight dependent mechanism. Furthermore, it inhibited tubular morphogenesis of MvECs propagated in collagen gels inside a dose and caspase eight dependent manner as a result of a mechanism that’s blocked by an antibody against the TSP one receptor on MvECs. These findings indicate that ABT 510 should be evaluated like a therapeutic alternative for individuals with malignant glioma.
ET 03. INDUCTION OF AUTOPHAGY IN MALIGNANT GLIOMA CELLS BY TELOMERE three OVERHANG Exact DNA OLIGONUCLEOTIDES Hiroshi Aoki,1 Eiji Iwado,one Mark S. Eller,2 Yasuko Kondo,one Keishi Fujiwara,1 Guang Zhi Li,two Kenneth R. Hess,3 Doris chloroxine R. Siwak,four Gordon B. Mills,4 Barbara A. Gilchrest,two and Seiji Kondo1,five,six, 1Department of Neurosurgery, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA, 2Department of Dermatology, Boston University College of Medicine, Boston, MA, USA, Departments of 3Biostatistics and Applied Mathematics and 4Molecular Therapeutics, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA, 5The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX, USA, and 6Department of Neurosurgery, Baylor School of Medicine, Houston, TX, USA Telomere three overhang specific DNA oligonucleotides induce cell death in cancer cells by mimicking telomere loop disruption.
Consequently, T oligos may perhaps be practical being a new therapeutic technique for different cancers. The objective of this research was to elucidate how T oligos exert antitumor results on human malignant glioma cells in

vitro and in vivo. We demon strated that T oligos inhibited the proliferation of malignant glioma cells as a result of induction of non apoptotic cell death and mitochondria hyperpo larization, whereas normal astrocytes had been resistant to T oligos. Tumor cells treated with T oligos showed autophagic features, with development of autophagic vacuoles and conversion of an autophagy related protein, microtubule associated protein 1 light chain three from type I to type II. A reverse phase protein microarray analysis revealed that treatment with T oligos inhibited the Akt/mammalian target of rapamycin pathway that’s associ ated with regulation of autophagy.