Urinary N-terminal crosslinking telopeptide of type I collagen (NTX) was measured with an electrochemiluminescent immunoassay on an automated machine (Vitros ECi, p38 MAPK inhibitor review Johnson and Johnson, Rochester, NY, USA). The intra- and interassay coefficients of

variation were below 7 and 6 %, respectively. The detection limit of the test was 4 nM, and the limit of quantitation was 22 nM. This measurement was corrected for creatinine (NTX/Cr). Serum C-terminal crosslinking telopeptide of type I collagen (CTX) was measured using an enzyme immunoassay kit (Serum CrossLaps®, Nordic Bioscience Diagnostics, Herlev, Denmark). The intra- and interassay coefficients of variation were below 8 and 6 %, respectively. The lower limit of detection was 0.044 ng/mL. Bone turnover marker assays were performed at a central laboratory (Synarc SAS, Lyon, France). The samples for the 24-month study visit were measured at a different VS-4718 solubility dmso time than the samples for all previous visits. Safety assessments Physical examinations were performed at baseline and after 12 and 24 months. Vital signs, concomitant medications, and adverse event reports were recorded at regular clinic visits throughout the study. Adverse event reports were captured using the Medical Dictionary for Regulatory Activities (MedDRA) system. Blood and urine samples for clinical chemistry and other standard laboratory measurements were collected at baseline and after 3, 6, 9, 12, 18, and 24 months

of treatment. GDC-0994 concentration Specimens were analyzed by Quintiles Laboratories

(Smyrna, GA, USA). Statistical analysis The primary endpoint analysis was a test of non-inferiority comparing the least squares mean percent change from baseline in lumbar spine BMD in the 150-mg once-a-month and 5-mg daily groups after 12 months. This test employed a predefined non-inferiority margin of 1.5 % and a one-sided type I error of 2.5 %. The results of this analysis have been published previously [6]. Secondary endpoints included the percent change from baseline in lumbar spine BMD at months 6 and 24, and at endpoint; the percent change from baseline in BMD of the total proximal femur, femoral neck, and femoral trochanter at months 6, 12, and 24, and at endpoint; the percentage of patients with new vertebral fractures at year 1 and 2; and the percent change from baseline in biochemical markers of bone turnover (NTX/Cr, CTX, and BALP) at months 3, 6, 12, and 24, and at endpoint. All data 17-DMAG (Alvespimycin) HCl reported here are based upon cumulative data collected over the entire 2-year treatment period. After 2 years of treatment, a non-inferiority analysis was performed based on the one-sided 97.5 % confidence interval (CI) for the difference in mean percent change from baseline to month 24 in lumbar spine BMD. The CIs were constructed using an ANOVA model with fixed effects for treatment and pooled investigative center. If the upper bound of the 97.5 % one-sided CI did not exceed 2.0 %, then the once-a-month treatment was considered non-inferior to the daily treatment.