There exist many inherent limitations of modeling a secreted bacterial virulence factor in vitro and of the mouse as a surrogate host for GAS infection studies. However, our studies do strongly suggest that the endogenous expression of EndoS may be redundant or dispensable for M1T1 GAS phagocyte resistance and pathogenicity, since targeted mutation of the other factors described above do yield clear attenuation of virulence phenotypes in similar in vitro and in vivo assay systems. Conversely, pretreatment of plasma containing antibodies against GAS with recombinant EndoS reduced opsonphagocytic killing of GAS, and heterologous overexpression of EndoS in a less virulent M49 GAS strain conferred

increased phagocyte resistance and increased lethality in the mouse infection model. These results suggest that high level www.selleckchem.com/products/Everolimus(RAD001).html expression or local accumulation of EndoS in tissues could contribute to virulence in certain GAS strain

backgrounds or infection scenarios, a subject that could merit future analysis in larger clinical or molecular epidemiologic surveys. EndoS is highly conserved among GAS serotypes and can also be found in Streptococcus equi and zooepidemicus [12]. Therefore, it was somewhat surprising that we could not detect a significant contribution to Rapamycin mouse GAS virulence in vivo. This may be due to the limitations of the mouse model used, and the expression levels of EndoS during the murine infection. The expression level of this enzyme during a human infection could have an impact on GAS immune cell killing resistance

but this remains to be investigated. The specificity of EndoS CSF-1R inhibitor activity towards IgG suggests that the enzyme may have an important role in the pathogenesis of GAS, yet to be discovered. Finally, whether or not GAS CYTH4 can effectively deploy this unique enzymatic activity targeted IgG N-glycosylation to promote its own survival in the host (as is intuitively appealing), the enzyme itself has already proven a promising lead biotherapeutic for treatment of antibody-mediated inflammatory pathologies [17, 25–29]. Conclusions We conclude that in a highly virulent M1T1 background, EndoS has no significant impact on GAS phagocyte resistance and pathogenicity. However, our overexpression experiments could indicate that local accumulation or high levels of expression of EndoS can contribute to virulence in certain GAS strains, or in other infection scenarios than the systemic infection model used in this study. Methods Bacterial strains and growth GAS strain 5448 (serotype M1T1, ndoS-positive) and GAS strain NZ131 (serotype M49, ndoS-negative) are well-characterized and were selected for use in this study [30, 31]. Escherichia coli MC1061 was used as cloning tool [32]. The streptococcal and E. coli strains were propagated on Todd-Hewitt agar (THA).