The episquamal side of the scale possesses concentric ridges (circuli) and grooves (radii) radiating from the central focus

to the edges AZD5363 of the scale. Each radius is covered by a dermal space with cells and blood vessels embedded within a loose matrix [3]. Scleroblasts synthesise and shape the scale matrix during ontogeny and regeneration [4]. The external layer is synthesised first, followed by the elasmodine layer, composed of types I and V collagen fibres in a plywood-like arrangement [5]. The collagens of the elasmodine layer are similar in arrangement to mammalian lamellar bone [6] and mineralise slowly from the external layer [7]. When a zebrafish scale is plucked from its scale pocket, formation of a new scale selleckchem is initiated immediately [8].

Already after two days, a new mineralised scale plate can be seen, but it takes up to four weeks for a new scale to grow to the size and thickness of the removed scale. As a consequence of this rapid reformation, the focus of early regenerating scales is less structured than that of ontogenetic scales. The typical grooves and radii appear late in scale regeneration, which is believed to be the result of basal plate remodelling [9] and [10]. Note that in this context, the term ‘ontogenetic’ scale is used for the scales that developed during the early ontogeny of the fish, in contrast to the scales that regenerate after plucking. The scale compartment constitutes a significant, readily accessible calcium source of fish as it can contain up to 20% of the total calcium in the body [11]. Fish withdraw calcium from their

scales in periods of high calcium demand, rather than from their axial skeleton as mammals do [12], [13] and [14]. However, mobilisation of scale calcium demands the same active and controlled mineralisation and demineralisation. Scales are covered with a monolayer of cells, originally called scleroblasts, on both the mineralised and unmineralised side [15]. More recent literature subdivides the scleroblasts in osteoblasts and osteoclasts, based on their scale forming and resorbing MycoClean Mycoplasma Removal Kit properties, respectively [16], [17] and [18]. This is substantiated by the classical osteoblast marker alkaline phosphatase (ALP), found in hyposquamal scleroblasts [19]. Both in mammals and in teleosts, staining of tartrate-resistant acid phosphatase (TRAcP) activity demonstrates bone surfaces that are being actively resorbed or have been resorbed [20]. Indeed, mononuclear and occasional multinuclear osteoclasts, positive for TRAcP but also the osteoclast marker cathepsin K, were found on the episquamal side of scales of different fish species [19] and [21]. Multinucleated osteoclasts resorbing the scale matrix have also been identified by means of electron microscopy [16] and [22]. Matrix degradation by osteoclasts is a key process in both normal bone turnover and the bone disease osteoporosis [23].