The antimicrobial material DualMesh Plus was utilized as positive control. learn more At every time, the inhibition zones were measured and the bacterial adhesion to the mesh surface quantified (sonication, scanning electron
microscopy). Cytotoxicity of the treatments was examined (alamarBlue) using rabbit skin fibroblasts. Results The largest zones of inhibition were created by allicin-chlorhexidine. Chlorhexidine was more effective than vancomycin, and allicin lost its effectiveness after 24 h. No bacteria adhered to the surface of the DualMesh Plus or the meshes soaked with vancomycin, chlorhexidine andallicin-chlorhexidine. On the contrary, saline and allicin allowed adherence of high loads of bacteria. Vancomycin had no toxic effects on fibroblasts, while allicin and chlorhexidine exerted high toxicity. Cytotoxicity was significantly reduced with the allicin-chlorhexidine combination. Conclusions The use of antiseptics such as chlorhexidine, alone or combined with others like allicin, could represent an adequate prophylactic selleck compound strategy to be used for hernia repair materials because soaking with these agents provides the mesh with similar antibacterial properties to those observed after soaking with vancomycin,
similar to the effect of DualMesh Plus.”
“Purpose. The purpose of this study was to evaluate early visual and refractive outcomes of Descemet’s stripping endothelial keratoplasty (DSAEK).\n\nMethod. Nine eyes of 7 male and 2 female patients, mean age 73 years, were treated with DSAEK. Eight had pseudophakic bullous keratopathy and 1 had advanced Fuch’s syndrome.
Patients were followed up for a mean of 11.4 months (range, 4-26). The DSAEK technique consisted of stripping Descemet’s membrane and endothelium from a recipient cornea and transplanting the posterior stroma and endothelium of a donor cornea using the Tan EndoGlide. The mean operating time was 61.89 minutes (standard deviation [SD], 9.3).\n\nResults. The median donor diameter was 8.375 mm (range, 8.25-8.75) and the mean donor thickness was 114.4 pm (range, 98-129). Mean preoperative sphere was -2.41 that changed postoperatively in -1.21. Mean endothelial cell loss was 25% (range, 23%-45%) at 6 months after surgery. Corneal pachymetry was reduced GW4869 from 796.6 mu m preoperatively to 535.5 mu m postoperatively. Best corrected vicual acuity (BCVA) was 20/40 or better postoperatively and exceeded the preoperative BCVA in all eyes. No graft failure was present. Two partial graft dislocations (1st and 5th postoperative day) were observed. One graft was successfully attached after reinjecting an air bubble (rebubbling) in the anterior chamber and the other graft was reattached with 4 10/0 nylon sutures after failed rebubbling.\n\nConclusions. The key to successful endothelial transplantation is the protection and preservation of as many donor endothelial cells as possible.