SMIC Pigmentk Rnchen. With this method, the behavior of pigment cells has been shown to vary by species of sea urchins. TCR Pathway For example, in the regular Ren Hemicentrotus pulcherrimus sea urchins, pigment cells leave the tip of the bowel may need during the sp Th gastrula stage and enter the plate animals, they begin in the aboral ectoderm toward the p Plants are migrating too. the prism stage, the pigment cells Feeder llig distributed in the aboral ectoderm. But Scaphechinus mirabilis irregular for take-urchins, pigment cells begin to migrate into the ectoderm of the p The animal before the beginning of gastrulation and are never found in the blastocoel. Remarkably, avoid the pigment cells of the two types

the migration.
Interestingly, the behavior of the pigment cells of the endoderm invagination defines pr sumtiver that occurs in two ways: by stages, and leads away. In general, the progressive invagination as there Regularia H. pulcherrimus, w During the continuous indentation is h Frequently Ritonavir in P. mirabilis Irregularia as observed, with some exceptions. For example, embryos of sea urchins have Toxopneustes regularly for take-pileolus further invagination, pigment cells migrate to the ectoderm of the aboral disk system to the p The animal in irregular Ren observed sea urchins. These variations of intussusception and the behavior of pigment cells that reflect the diversity of different species of sea urchins. Unlike prime Ren mesenchymal cells, SMCs have an inductive signal from Delta for their specification.
Most of the descendants of vegetal blastomeres is veg2 receive this signal via inductive Notch. Recently it was shown that Nodal, in Mutma Lichen oral ectoderm is expressed, is also involved in the description of CML. However, the mechanisms of SMC specification have been studied only in a limited number of species of sea urchins, and signals, which can be used for the specification SCM vary between species of sea urchins. By examining the relationship between the way the intussusception and the behavior of pigment cells, we found a new population of fluorescent cells in the keyhole sand dollars Astriclypeus Manni. These cells are fluorescent under UV light, but not accumulate Pigmentk Rnchen and remain transparent, as opposed to pigment cells. In addition, unlike pigment cells, these cells were detected in the oral ectoderm.
In this study the behavior of these cells at the stage of the connective tissue was observed to pluteus blastula stage. To determine whether Notch is involved in the description of these fluorescent cells, the embryos with 2 NL alanyl phenyl] glycine 1.1 dimethylethyl ester, an inhibitor of gamma-secretase were treated. To determine whether Nodal signaling is important for the specification of these cells, the embryos with SB431542, a specific inhibitor of transforming growth factor-beta superfamily receptor type 1 were treated. In addition, the time of specification of these fluorescent cells by pulse-treatment embryos with DAPT and quantification of the number of cells examined. Our results show that these cells are a new population of CML has not been observed in other species of sea urchins. Materials and Methods Animals Adults and embryos of the sand dollar barrier A. Manni were on a sandy beach, only collected