structure in Fig  1c) Table 1 1H hfcs [MHz] of P•+ in wild-type r

structure in Fig. 1c) Table 1 1H hfcs [MHz] of P•+ in wild-type reaction centers from Rb. sphaeroides and mutants at pH 8.0 with (tentative) assignments,

ratios and sums of hfcs, and EPR linewidths   Wild typea Wild typeb ND(L170) ND(M199) A(12 L 1 ) 5.64 5.57 [5.43] 6.82 [7.00] 3.54 A(2 L 1 ) 4.01 3.90 [3.86] 4.98 2.59 A(12 M 1 ) 3.10 3.21 ~1.4 6.32 A(2 M 1 ) 1.36 1.30 ~0.58 (calc.) 2.59 β L (strong) 9.70/8.66 9.51/8.52 13.28/11.52   β M (strong)       12.61/11.24 A(12 L 1 )/A(2 L 1 ) 1.41 1.43 1.37 1.37 A(12 M 1 )/A(2 M 1 ) 2.28 2.47 2.4(from WT) 2.44 ΣA 14.11 13.98 ~13.78 15.04 ρ L 0.68 0.68 ~0.86 0.41 ρ L/ρ M 2.13 2.13 ~6.14 0.69 ΔBpp [G] 9.6 9.6 11.0 10.1 aWild type Rb. sphaeroides 2.4.1 grown under photosynthetic Selleck Gemcitabine conditions bWild type Rb. sphaeroides with hepta-histidine tag (WT-H7) grown under non-photosynthetic conditions ΔBpp [G] is the peak-to-peak gaussian envelope EPR line width; the INCB28060 concentration error is ±0.2 G Error for methyl group hfcs is ±70 kHz, for other β-proton hfcs ±120 kHz, for the double mutant the errors are higher a iso values given in square brackets are from frozen solution Q-band ENDOR experiments ΣA is the sum of A(12 L 1 ), A(2 L 1 ), A(12 M 1 ), and A(2 M 1 ) ρ L is the fraction of spin density on ρ L as measured by [A(12 L 1 ) + A(2 L 1 )]/[A(12 L 1 ) + A(2 L 1 ) + A(12 M 1 ) + A(2 M 1 )] ρ L/ρ M is the ratio of the spin densities on PL and PM as measured by

[A(12 L 1 ) + A(2 L 1 )]/[A(12 M 1 ) + A(2 M 1 )] P•+ in mutant RCs Since the mutants show pronounced pH dependences of the P/P•+ midpoint potential and electron transfer rates, the spectra were measured at three different pH values, 6.5, 8.0, and 9.5. Based upon previous studies (Haffa et al. 2002; 2003; 2004; Williams et al. 2001),

comparison of the spectra of the mutants at different pH values should show the effect selleck kinase inhibitor of changes in the protonation, or charge, of the introduced residue. At any given pH, the deprotonated and protonated forms of the residue will be in equilibrium with a ratio determined by the pK a value. If the protonation and deprotonation process is fast compared to the EPR/TRIPLE timescale, only an averaged single species with a shifted spin density distribution will be observed. Considering that already the Special TRIPLE spectra of one species is rather complicated due to the large number of coupled protons, it P505-15 research buy becomes difficult to disentangle these spectra containing contributions from two P•+ species.

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