Ostate cancer PC3 cells. In addition, we also JAK Inhibitors examined influence whether Z ligustilide k nnte The cytotoxicity t of L Dopa in PC12 cells or not. In particular, we have no significant cytotoxicity T, where L Dopa was administered extracellular Ren Nor the synergistic toxicity t at L dopa administered was observed together with Z ligustilide in PC12 cells. Overall, these results suggest that the synergistic cytotoxicity t is of Z ligustilide and dopamine specific dopaminergic cells. Glutathione is an endogenous antioxidant necessary for intracellular Ren redox Hom And cellular homeostasis Ren resistance against oxidative insults. It is known that various oxidized derivatives react spontaneously with dopamine reduced GSH or in the presence of glutathione S-transferase. TPS supply is the first step in the detoxification and excretion of toxic electrophiles in cellular Re defense. Consequently, the supply GST generally as a protective mechanism. However, recent studies suggest that dopamine thioether derivatives have k Can potent neurotoxins in the brain. Not to hnen the toxic dopamine thioether mentioned, The results of this study at least one drop hunter that dopamine quinone consumed massively reduced GSH far entered Ing a sharp drop in the level of intracellular Ren GSH. In addition, we have recently shown that Z ligustilide transient decrease in GSH caused in a manner dependent on the concentration Dependent. Curiously, to Z ligustilide and dopamine in synergistic combination, the level of intracellular Ren GSH. Overall, the results of this study is that Ersch Pfung the intracellular Ren GSH convergent another mechanism may underlie the synergistic cytotoxicity t of Z ligustilide and dopamine represent. To assess the contribution of the formation of ROS and GSH depletion to determine, we have examined thiol-containing antioxidants and Herk.
Mmlichen antioxidants to inhibit the Daunorubicin cytotoxicity t Z ligustilide and dopamine. We found that the antioxidants NAC and GSH thiol largely suppressed the cytotoxicity t of Z ligustilide and dopamine, w While the non-thiol antioxidant ascorbic Acid and Trolox showed only a marginal activity t. This Best term, our results the r The key level of intracellular Ren GSH against the synergistic cytotoxicity t of Z ligustilide and dopamine. Lim and Zhou recently proposed several mechanisms by which GSH protects dopaminergic cells against the toxicity of t induced by dopamine. GSH and other reducing agents inhibit the oxidation of dopamine to dopamine quinone toxicity t. On the other c T, GSH inhibits the aggregation of various GSH conjugates are violating identified in polymeric structures similar to the protein conjugates in Lewy-K rpern of Parkinson’s disease. Therefore it is important that the balance between the oxidation of dopamine and GSH maintain protective layer. In Similar way, NAC, the protective effect by producing a reducing agent and a precursor Shore for the biosynthesis of intracellular GSH. After addition of dopamine, was the color of the cell culture medium was found to be brown-orange, indicating that dopamine was oxidized metabolites in various quinine. We found that two NAC and GSH inhibited the color Change of the incubation medium. Taken together, we argue that both NAC and GSH inhibit the oxidation of dopamine and perhaps also the polymerization of dopamine-quinone-GSH conjugates, drawings.