Pplays a role Ma Have gebliche participation in anaphase chromosome 23 movement.21 others reported that depletion of MCAK lags in CHO cells caused the appearance of chromosomes may need during the GSK1070916 Aurora Kinase inhibitor anaphase, a result that as evidence that separation of sister chromatid help MCAK microtubule destabilizing at the kinetochores.4 It is should be noted however, that the chromosomes k can errors occur sp th orientation was interpreted that anaphase24 occur and are therefore not a good marker for M shortcomings, which are specific for chromosome anaphase movement . In addition, term k best Not nnten further studies, An R MCAK during anaphase A. movement.
6 r playing For the children I like protein may need during the anaphase was also in insects, which was proposed KLP59C reported to be responsible for 60% of the rate of anaphase chromosome movement in Drosophila embryos.5 Similar to the situation of mammalian cells in S, But has RNAi GSK461364 929095-18-1 studies in S2 insect cells, this observation is not best term. 25 The participation of MCAK in anaphase thus remained an open question. Because we have no pure populations of cells in metaphase anaphase or prepare, k We can not measure exactly how many biochemical degradation after MCAK in metaphase remains, but even with an imperfect Synchronit t of the drop seems significant. In addition, we have the best visual Confirmation of MCAK in the sp Later stages of mitosis are reduced, and another recent study reported reduced MCAK F K staining in MCF-7 and T47D cells.
8 Yet We can not exclusively s, that the small amount that is able to catalyze the metaphase to MCAK depolymerization of microtubules and anaphase chromosome movement. It seems illogical, but that the target cell destruction Tion MCAK at a time when his need for the size Th is. Instead, we favor the idea that the Head T ACTION of MCAK may need during the events that occur at metaphase. 6 The destruction Tion of MCAK may need during the mitotic arrest in the M Possibility that their pension Pr Presence may need during the anaphase may st Liked rdern t than f, The sister chromatid segregation. Unfortunately, this is M Possibility is not directly verifiable. Inhibitors, which prevent k Nnten, MCAK phosphorylation stabilize the protein, then put But nonspecific and k Can inhibit mitotic progression is that we saw in the case of the Aurora kinase inhibitor ZM447439 B.
In order to treat it correctly, if MCAK beautiful dlichen effects in anaphase have k nnte, it will be necessary to have sequences in MCAK its degradation involved and mutate these sites in order to stabilize the protein. Although we judge can not know whether the presence of specific high MCAK w While would anaphase an adverse effect on chromosome movement anaphase, then put Ahead we say that one of the m Aligned sequences of degradation inhibition MCAK one obtains Is hter content cell in the protein by mitosis, which would be detrimental to the mitotic growth. Previous studies have shown that leads to overexpression of MCAK for the formation of abnormal spindles and prometaphase arrest.4 have shown 10 Our studies show that overexpression has two times MCAK has no effect on mitosis or growth of cells, but only 6-fold overexpression Ganguly and Al Page