coli K12: MG1655 and W3110 (both derived from W1485 https://www.selleckchem.com/products/KU-55933.html approximately 40 years ago [98]), and DH10B which was constructed by

a series of genetic manipulations [99]. Each of these three substrains encode 89 lipoproteins found in both other substrains (Additional file 4). Four additional lipoproteins are detected in DH10B (BorD, CusC, RlpA and RzoD) and are second copies lipoprotein genes, present in the 113-kb tandemly repeated region of the chromosome (Figure 8B, coordinates 514341 to 627601, [99]), and strain DH10B contains one gene encoding the Rz1 proline-rich lipoprotein from bacteriophage lambda absent from the two other substrains. Lipoprotein YghJ, that shares 64% homology with V. cholerae virulence-associated accessory colonization factor AcfD [100], is absent from the DH10B genome annotation. However, comparative genomic analysis shows that a yghJ locus could be annotated in this strain but corresponds to a pseudogene https://www.selleckchem.com/products/a-1210477.html caused by a frameshift event (Figure 8C). YfbK was also overlooked in the DH10B annotation process but in this case, the gene is intact. Finally, differences between lipoprotein prediction results concerning YafY, YfiM and YmbA are due to erroneous N-terminus predictions. YafY in DH10B was predicted to be a lipoprotein due to the N-terminal 17 aa-long type II signal peptide and was published as a new inner membrane lipoprotein [101]. In substrains MG1655 and WS3110, the original annotation

fused the yafY loci with its upstream pseudogene ykfK (137 N-terminal aa longer). The presumed Selleck MCC 950 start codons of YfiM and YmbA in MG1655 were recently changed by adding 17 (lrilfvcsllllsgcsh) and 5 (mkkwl) N-terminal amino acids, respectively (PMC1325200). These modifications substantially affect the prediction of their subcellular localization. Inspection Inositol monophosphatase 1 of the genomic sequences of the two other substrains leads to equivalent changes such that YfiM and YmbA in all three substrains are now predicted to be lipoproteins.

In conclusion, using CoBaltDB to compare lipoproteomes between substrains, we were able to detect genomic events as well as “”annotation”" errors. After correction, we can conclude that the three E. coli K12 substrains have 93 lipoproteins in common; that one locus whose function is related to virulence has been transformed into a pseudogene in DH10B; and that DH10B contains five additional lipoproteins due to duplication events and to the presence of prophages absent from the other two substrains (Figure 8D). Figure 8 Using CoBaltDB in comparative proteomics. Example of E. coli K12 substrains lipoproteomes. 4-Using CoBaltDB to improve the classification of orthologous and paralogous proteins Protein function is generally related to its subcellular compartment, so orthologous proteins are expected, in most cases, to be in the same subcellular location. Consequently, inconsistencies of location predictions between orthologs potentially indicate distinct functional subclasses.