Minipericardiotomy a 1 cm was cleared at the front zipper and a combined 6 Fr conductance catheter was introduced pressure volume ju a purse-string suture R is positioned below the pulmonary valve and RV apex.12 A second incision 1 cm was in the pericardium to the RA Anh Ngsel made, and a catheter combined Fr 5 PV conductivity Ability on the long axis is positioned to the right atrium, so that the peak in BMS-540215 the IVC junction.11 RA, RA and RV 12.13 photovoltaic rested catheters Questions were two signal conditioners processors.14 data capture data have been connected in the inflated state of equilibrium with the PA in order to simulate conditions as they appear recorded in chronic pulmonary hypertension. W During each cycle of data collection, BE, RAP, RVP, aortic pressure, flow SVC, IVC and RA and RV conductance signals were recorded at 200 Hz and processed with software con Custom and After data was obtained steady, slow progressive cava occlusion was performed generate RA and RV PV loop to a wide range of physiological filling pressures.
Once the data is collected, an intravenous Se 10mg/hr diltiazem was started. After a stabilization period of 20 minutes, the data were analyzed with the PA group always aufgebl Collected Epothilone B ht, simulate a non-responders CWB. After all, the PA cuff was released to unload the right ventricle and standardized data collection was repeated. Gem the definition of clinical responders CWB ver we ffentlichten PA-band results in a decrease of 20% to 30% of the average PA and RV pressure was monitored. At the end of the experiment, the animals were eingeschl Tert use pentothal followed by potassium chloride, and the correct positioning of the catheter was best CONFIRMS.
All animals were again U humane care in accordance with the ground protect Protection of laboratory animals by the National Society for Medical Research and the Guide for the Care and Use of Laboratory Animals by the National Academy of prepared formulated Sciences and ver Ffentlicht of the National Institutes of Health. The study was approved by the Washington University School of Medicine Animal Studies and conducted by Washington University policy. The authors w hlten To remain well-established model for chronic canine purposes of this study because the complex instruments necessary to assess simultaneously RV and biomechanics of PR at different levels of RV afterload currently prohibits the use of a rodent model. Data analysis RA elastance was calculated by the right atrial systolic pressure volume relationship, as previously described in detail.
12, 15 Described similar, the RA is static stiffness is defined as the slope of the end diastolic right atrial pressure-volume relationship. RV elastance and stiffness Similar mathematical methods were used RV elastance and the steepness of the slope of the ESPVR EDPVR.16 RV and RV, rechtsventrikul Higher pressure coupling 17 compute effective arterial compliance beautiful PROTECTED the pulmonary vascular System and RV afterload systolic function VPR beaches determination and systolic RVPES Ea / SV.17 effective arterial compliance is an Ann approximation to the station safe state maximum pulmonalvaskul Ren impedance. The coupling rate rechtsventrikul Ren pressure Ea / EDC was then calculated, which reflects the condition of correspondence between the elastance right ventricle and the pulmonary artery, the pressure in charge volume.