It KAS 6.1 MIP1 cells within two weeks after the injection cell. This model does not apply to Knochenzerst Tion AT9283 cause localized, but diffuse osteoporosis due to i in multiple myeloma. Multiple Myeloma mice were studies on the effectiveness approximately 4-week-old female CB17 SCID-M Obtained from Harlan Sprague Dawley Industries, and in a barrier facility under a 00:00 light / dark cycle with access ad libitum food and water. about 24 hours before tumor cell injection, mice were anesthetized UN M with C sium cG 250 with Mark 25th January-gamma emitter. KAS 6/1 myeloma cells were fixed MIP1 in the tail of M Mice on day 0, injected well. The compounds and the tests were based on the results of our studies, the safety and efficacy of breast cancer and included 0.
04, 0.4, or 4.0 g / day MBC 29, 11 and 1, 0, 04 or 4.0 g / day etidronate, AraC, AraCetidronate, FUR, FU, and zoledronate. PBS was included as controls The vehicle. Since the compounds of contr The Reinholz et al. Page 5 bones. Author manuscript, increases available in PMC 2011 1 July. PA Author Manuscript NIH-PA Author Manuscript ABT-751 NIH-PA Author Manuscript NIH were not discussed at the mid dose, the effects of 0.40 g / day dose in Table S3 in further detail. T Resembled sc administration of the compounds began resuspended in sterile PBS two weeks after the injection of cells at the time of ANF Nglichen bone loss, until the necessary sacrifice of animals.
Mice get Were switched on when they do not get more food and water were on their own, have lost their righting reflex, lost the excess weight, or if the sustained L Occurred hmung. Were analyzed in bone density regularly In anesthetized mice ig M, Carried out every two weeks from the time of injection of cells until the day of sacrifice necessary with dual R Measured by an energy ntgenabsorptiometrie PIXImus lunar instrument. At the time of sacrifice, the animals were eingeschl Tert gross pathology and the different organs for each type of metastatic tumor burden was examined. On the macroscopic assessment, we did not observe any tumors in the organs tested. The statistical analysis of parametric tests may need during the tests of normality T and equal variance were performed assumed and nonparametric tests were performed on failed tests of normality T.
Parametric multiple comparison groups were analyzed using one-way ANOVA with post hoc Tukey test and nonparametric multiple group comparisons were made with lengths ANOVA, Kruskal Wallis one-way on the R With Dunn post hoc tests. Parametric and nonparametric comparisons of the two groups were performed with the Student t-test and Wilcoxon tests respectively. Likelihood ratio Chi-square analyzes were used to test for significant differences in the H FREQUENCY of bone metastases and the loss of bone density between multiple groups. Fisher was, s exact tests are used to test for significant differences in the H FREQUENCY of bone metastases and loss of bone density between the two groups. Two-way analysis of variance were analyzed used to test differences between the effects of five concentrations and tested compounds on the in vitro proliferation of cells of several myeloma cell lines. Rank tests of survival was plotted using Kaplan-Meier and log were used to evaluate the significance of the grouping factor. Multiple comparison tests, p values of 0.05 used to justify further investigation