As in

As in maize kernels the greater part of phytic acid (and thus of metal ions) is concentrated in the embryo, its antioxidant action may

be of particular relevance in this crop.”
“Background- Long QT syndrome (LQTS) is the most common cardiac channelopathy with 15 elucidated LQTS-susceptibility genes. Approximately 20% of LQTS cases remain genetically elusive.

Methods and Results- We combined whole-exome sequencing and bioinformatic/systems biology to identify the pathogenic substrate responsible for nonsyndromic, genotype-negative, autosomal dominant LQTS in a multigenerational pedigree, and we established the spectrum and prevalence of variants in the elucidated gene among a cohort of 102 unrelated patients with “”genotype-negative/phenotype-positive”" LQTS. Whole-exome sequencing was used on 3 members within a genotype-negative/phenotype-positive family. Genomic triangulation combined with bioinformatic tools and ranking algorithms led to the identification of a CACNA1C mutation. This mutation, Pro857Arg-CACNA1C, cosegregated with the disease within the pedigree, was ranked by 3 disease-network algorithms

as the most probable LQTS-susceptibility gene and involves a conserved residue localizing to the proline, gltamic acid, serine, and threonine (PEST) domain in the II-III linker. Functional studies reveal that Pro857Arg-CACNA1C leads to a gain of function with increased I-Ca,I-L and increased surface membrane expression of the channel compared to wild type. Subsequent mutational analysis identified 3 additional variants within CACNA1C in our cohort Alvocidib chemical structure of 102 unrelated cases of

genotype-negative/phenotype-positive LQTS. Two of these variants also involve PF-3084014 conserved residues within Ca(v)1.2′s PEST domain.

Conclusions- This study provides evidence that coupling whole-exome sequencing and bioinformatic/systems biology is an effective strategy for the identification of potential disease-causing genes/mutations. The identification of a functional CACNA1C mutation cosegregating with disease in a single pedigree suggests that CACNA1C perturbations may underlie autosomal dominant LQTS in the absence of Timothy syndrome.”
“Large fragments of a transplanted lung from living lung recipients are rarely available. The case presented is that of a patient who underwent lingulectomy after a gunshot trauma. We describe the patchy panel of histological findings encountered in the resected specimen, ranging from normal lung to zones of acute and chronic rejection. Such contrasting findings have already been described, but should prompt us to exercise caution when interpreting histological results of routine transbronchial biopsies.”
“Background: This paper examines correlates of needle and other equipment sharing among injecting drug users (IDU) in regular heterosexual relationships.

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