Although Romosinuano have a lower respiration rate and ruminal temperature than Angus, they share a similar pattern of respiration rate adaptation from early to late summer periods. (C) 2011 Elsevier Ltd. All rights reserved.”
“MS-based proteomics produces large amounts

of mass spectra that require processing, identification and possibly quantification before interpretation can be undertaken. High-throughput studies require automation of these various steps, and management of the data in association with the results obtained. We here present ms_lims (http://genesis.UGent.be/ms_lims), a freely available, open-source system based on a central database to automate data management and processing in MS-driven proteomics Elafibranor solubility dmso analyses.”
“Despite the current clinical use of boronophenylalanine-fructose (BPA-f), as radiosensitizer, in BNCT application for brain tumors, still remains to be determined the safety dose of this agent. We evaluated the potential risk of primary BPA-f toxicity before neutronic irradiation at different concentrations (0-100 mu g B-eq/ml) after short- and long-term exposure

(4-48 h and 7-10 days), using a battery of tests (i.e. MTT assay, calcein-AM/Propidium Iodide staining, clonogenic test) in CNS cell models (D384 and SH-SY5Y), and non-neuronal primary human fibroblasts (F26). MTT data showed: (i) no cytotoxic effects after short-term exposure (4 h) to any of BPA-f concentrations tested in all cell models;

(ii) dose- and time-dependent mitochondrial S3I-201 nmr activity impairment in D384 and SH-SY5Y cells only (with 60% and 40% cell death in D384 and SH-SY5Y, respectively, after 48 h exposure to BPA-f 100 mu g B-eq/ml). By Calcein-AM/PI staining, BPA-f treatment was specific toward SH-SY5Y cells only: a dose-dependent cell density reduction was observed, with a more pronounced effect after 48 h exposure (15-40% at doses ranging 20-100 mu g B-eq/ml). Clonogenic data revealed dose-dependent decrease of cell proliferative capacity in all cell lines, still the SH-SY5Y cells were the most sensitive ones: the lowest dose (20 mu g B-eq/ml) produced 90% cell decrease. These results indicate dose- and time-dependent cytotoxic effects of BPA-f, with CNS cells showing a lower tolerance compared selleck kinase inhibitor to fibroblasts. Long-term exposure to BPA-f compromised the proliferative capacity regardless of cell model type (cell sensitivity being SH-SY5Y > D384 > F26). In short-time exposure, BPA-f exhibits a safe dosage up to 40 mu g B-eq/ml for the viability of CNS cell lines. (c) 2012 Elsevier Inc. All rights reserved.”
“The aim of this study was to investigate the diurnal variation in core temperature in aluminium shift-workers exposed to hot ambient conditions. Core temperature was continuously recorded via an ingestible radio-telemetry thermistor in 29 shift-workers.