(TIFF 134 KB) Additional file 3: IFM Adhesion inhibition assay with DAPI staining. M. pneumoniae were pre-incubated with monospecific antibodies in different dilutions (1 in 50, 1 in 100, 1 in 200, 1 in 500) before infection of the HEp-2 cells. M. pneumoniae infected HEp-2 cells were stained with Evans blue (red) and DAPI (blue). The M. pneumoniae microcolonies

attached to HEp-2 cells ABT-263 cost are detected by (a-d) Pab (rP1-I), (f-i) Pab (rP1-IV) and (e & j) pre-bleed rabbit sera with FITC conjugated secondary antibody (green fluorescence). The nuclear material of M. pneumoniae microcolonies were not detected by DAPI staining. (TIFF 587 KB) Additional file 4: Comparative study of Immunodominant see more region(s) of P1 protein of M. pneumoniae . Comparison of the immunodominant regions identified in the present study and a number of previous studies. ★ Immunogenic region, aa Amino acid, nt Nucleotide. (TIFF 33 KB) Additional file 5: Comparative study of cytadherence region(s) of P1 protein

of M. pneumoniae . Comparison of cytadherence regions identified in the present study and a number of previous studies. ★ Cytadherence region, aa Amino acid, nt Nucleotide. (TIFF 36 KB) References 1. Razin S, Yogev D, Naot Y: Molecular biology and pathogenicity of mycoplasmas. Microbiol Rev 1998, 63:1094–1156. 2. Razin S, Kahane I, Banai M, Bredt W: Adhesion of mycoplasmas to eukaryotic cells. Ciba Found Symp 1981, 80:98–118.PubMed 3. Clyde WA Jr: Clinical overview of typical Mycoplasma pneumoniae LY3023414 ic50 infections. Clin Infect Dis 1993,17(Suppl 1):S32-S36.PubMed 4. Hu PC, Collier AM, Edoxaban Baseman JB: Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium. J Exp Med 1977,145(5):1328–1343.PubMedCrossRef 5. Chaudhry R, Tabassum I, Kapoor L, Chhabra A, Sharma N, Broor S: A fulminant case of acute respiratory distress syndrome associated with Mycoplasma pneumoniae infection. Indian J Pathol Microbiol 2010,53(3):555–557.PubMedCrossRef 6. Sharma MB, Chaudhry R, Tabassum

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