5 ml of assay buffer. After the resuspension
of cells in scintillation fluid (Rotiszinth, Roth, Germany) the radioactivity of the sample was counted in a scintillation counter (Beckman, Krefeld, Germany). Acknowledgements The authors would like to thank Lothar Eggeling (Jülich) PU-H71 manufacturer for discussions during the initial phase of the project. We acknowledge support of the publication fee by Deutsche Forschungsgemeinschaft and the Open Access Publication Funds of Bielefeld University. References 1. Said HM: Biotin: the forgotten vitamin. Am J Clin Nutr 2002, 75:179–180.PubMed 2. Streit WR, Entcheva P: Biotin in microbes, the genes involved in its biosynthesis, its biochemical role and
perspectives for biotechnological production. Appl Microbiol Biotechnol 2003, 61:21–31.PubMed 3. Lin S, Cronan JE: Closing in on complete pathways of biotin biosynthesis. Mol Biosyst 2011, 7:1811–1821.PubMedCrossRef 4. Bower S, Perkins JB, Yocum RR, Howitt CL, Rahaim P, Pero J: Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon. J Bacteriol 1996, 178:4122–4130.PubMed 5. Lin S, Hanson RE, Cronan JE: Biotin synthesis begins by selleck chemical hijacking the fatty acid synthetic pathway. Nat Chem Biol 2010, TSA HDAC in vitro 6:682–688.PubMedCrossRef 6. Rodionov DA, Dubchak I, Arkin A, Alm E, Gelfand MS: Reconstruction of regulatory and metabolic pathways in metal-reducing delta-proteobacteria. Genome Biol 2004, 5:R90.PubMedCrossRef 7. Harrison FH, Harwood CS: The pimFABCD operon from Rhodopseudomonas palustris mediates dicarboxylic acid degradation and participates in anaerobic benzoate degradation.
Microbiology 2005, 151:727–736.PubMedCrossRef ADP ribosylation factor 8. Udaka S: Screening method for microorganisms accumulating metabolites and its use in the isolation of Micrococcus glutamicus . J Bacteriol 1960, 79:745–755. 9. Hatakeyama K, Hohama K, Vertes AA, Kobayashi M, Kurusu Y, Yukawa H: Genomic organization of the biotin biosynthetic genes of coryneform bacteria: cloning and sequencing of the bio – bio genes from Brevibacterium flavum . DNA Seq 1993, 4:177–184.PubMed 10. Hatakeyama K, Kobayashi M, Yukawa H: Analysis of biotin biosynthesis pathway in coryneform bacteria Brevibacterium flavum . Methods Enzymol 1997, 279:339–348.PubMedCrossRef 11. Hatakeyama K, Kohama K, Vertes AA, Kobayashi M, Kurusu Y, Yukawa H: Analysis of the biotin biosynthesis pathway in coryneform bacteria: cloning and sequencing of the bio gene from Brevibacterium flavum . DNA Seq 1993, 4:87–93.PubMed 12. Okumura S, Tsugawa R, Tsunoda T, Morisaki S: Studies on L-glutamic acid fermentation. Part II. Activities of various pelargonic acid compounds to promote fermentation. J Agric Chem Soc 1962, 36:204–211. 13.