46/5.57 18141/20000 ↑1.00 – Cytoplasmic L – Replication, recombination and repair 51 gi|222084927 ATP-dependent RNA helicase protein Agrobacterium radiobacter 9.17/5.36 69955/67000 2.29 ± 0.14 0.001 Cytoplasmic Poorly characterized R – General function www.selleckchem.com/products/gsk1120212-jtp-74057.html prediction only 52 gi|222086102 sufC FeS assembly ATPase SufC Agrobacterium radiobacter 5.08/4.95 27375/32000
↑1.00 – Inner Membrane 53 gi|222082138 cpo Chloride peroxidase protein Agrobacterium radiobacter 7.88/6.37 34965/32000 1.59 ± 0.02 0.001 Periplasmic 54 gi|186472508 wrbA Flavoprotein WrbA Burkholderia phymatum 6.19/5.91 20930/26000 2.58 ± 0.14 0.001 Cytoplasmic 55 gi|170699364 NADPH-dependent FMN reductase Burkholderia ambifaria
6.71/6.31 8539/17000 2.03 ± 0.19 0.002 Periplasmic 56 gi|194431754 dkgA 2,5-diketo-D-gluconic acid reductase A Shigella dysenteriae 6.22/5.15 19399/23000 1.34 ± 0.21 0.002 Cytoplasmic 57 gi|222085370 Ferredoxin reductase protein Agrobacterium radiobacter 5.88/5.65 43777/53000 1.48 ± 0.12 0.003 Cytoplasmic S – Function Unknown 58 gi|222149801 Hypothetical protein Avi_3814 Agrobacterium vitis 5.03/5.01 24632/29000 1.42 ± 0.34 0.033 Periplasmic NO related COG 59 gi|209547526 Hypothetical protein Rleg2_5527 KU-57788 order Rhizobium leguminosarum 6.02/5.89 33584/44000 1.57 ± 0.13 0.002 Cytoplasmic 1Theoretical/Experimental values. Da: Daltons. 2↑1.00 in the fold change ratio means that the protein was only identified in the experimental condition (35°C). Matched peptides masses and MS/MS combined results are available in PRIDE ( http://ebi.ac.uk/pride/) under the experiment accession number 14817. Among the differentially expressed proteins, twenty-five were related to metabolic functions, the majority of them associated with amino acid transport and metabolism (group E) (Table
1), corroborating the proteomic reference map of Bradyrhizobium japonicum strain CPAC 15, a microsymbiont of soybean [22], Cediranib (AZD2171) and indicating high metabolic activity even under stressful conditions. Also within this category, it is worth mentioning that NocP, an opine permease ATP-binding protein, was differentially expressed under high temperature. Opine is a compound released by crown-gall tumors produced by Agrobacterium (=Rhizobium) [23], and genes related to its metabolism were detected in the draft genome of PRF 81 and now confirmed at the translational level in our study. Putative genes related to rhizopine metabolism (an opine-like compound) were reported in R. tropici for the first time by our research group [12]. The ability to catabolize rhizopine appears to enhance the rate at which a strain is able to form nodules when it is in competition with a strain that is unable to catabolize a rhizopine. The mechanism responsible for this enhanced symbiotic ability is still unclear [24].