However, there are strategies ensuring that ion suppression can be identified and ameliorated (the real risk Tubacin clinical is lack of awareness that a problem exists and exaggerated claims by users). Another difficulty that I have found in my own laboratory with LC�CMS, especially when using an electrospray ionization (ESI) interface, is that after a few days of intense sample analysis, a fall off is observed in the sensitivity of the method. The problem is that the ESI needle gets dirty; cleaning of the needle and skimmer region rectifies the problem. Again, this is a shortcoming of LC�CMS that is not, I find, discussed broadly. I also feel that the complexity of the MS detector (especially when things go wrong) can lead to protracted down time when compared with the UV/DAD.
Do not misunderstand me, I am an advocate of LC�CMS in the pharmaceutical industry, and as a research tool, I find it indispensable but I predict that we will see the persistence of LC�CUV/VIS and DAD methodologies for many years to come. As sometimes what is adequate and simple is completely fit for purpose.
Ethacridine lactate [Figure 1], 2-ethoxy-6,9-diaminoacridine monolactate monohydrate (British Pharmacopoeia, 2005), also known as rivanol has been employed as a potent anti-microbial agent from the penicillin era and in various other tests on antigens too. In addition, it is a drug commonly used for second trimester termination of pregnancy which has associated with the lowest rate of complication. Ethacridine lactate as an abortifacient is found to be safer and better tolerated than 20% hypertonic saline.
The drug is official in British Pharmacopeia and Martindale. The literature survey revealed that one HPLC method is reported for ethacridine lactate in human plasma and one SP-HPLC method is developed. There are no spectrophotometric Entinostat methods reported on ethacridine lactate in pharmaceutical dosage form. Therefore, the main objective of this work is to develop a simple, selective, accurate, and precise spectrophotometric method for ethacridine lactate. The second objective is to validate the method as per the ICH guidelines.[5�C7] Figure 1 Chemical structure of ethacridine lactate MATERIALS AND METHODS Chemicals Ethacridine lactate is obtained from Venus Remedies Ltd., Haryana, India as a gift sample. Methanol (HPLC Grade) was purchased from Merck (India) Ltd., Worli, Mumbai, India. Ethacridine lactate solution infusion was purchased from Indian market, containing ethacridine lactate 1 mg per 100 ml. Instrumentation conditions Analysis was performed on UV-visible spectrophotometer Schimazdu 2450. Before analysis, the sample solution was filtered through a 0.