, 2010; Balenci et al., 2009). The hydroxyl radicals detection is performed by monitoring the NDMA characteristic band at 440 nm on the electronic spectra. Generation of the ˙OH radicals causes the decrease in the intensity of this band and can be measured in {Selleck Anti-cancer Compound Library|Selleck Anticancer Compound Library|Selleck Anti-cancer Compound Library|Selleck Anticancer Compound Library|Selleckchem Anti-cancer Compound Library|Selleckchem Anticancer Compound Library|Selleckchem Anti-cancer Compound Library|Selleckchem Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|buy Anti-cancer Compound Library|Anti-cancer Compound Library ic50|Anti-cancer Compound Library price|Anti-cancer Compound Library cost|Anti-cancer Compound Library solubility dmso|Anti-cancer Compound Library purchase|Anti-cancer Compound Library manufacturer|Anti-cancer Compound Library research buy|Anti-cancer Compound Library order|Anti-cancer Compound Library mouse|Anti-cancer Compound Library chemical structure|Anti-cancer Compound Library mw|Anti-cancer Compound Library molecular weight|Anti-cancer Compound Library datasheet|Anti-cancer Compound Library supplier|Anti-cancer Compound Library in vitro|Anti-cancer Compound Library cell line|Anti-cancer Compound Library concentration|Anti-cancer Compound Library nmr|Anti-cancer Compound Library in vivo|Anti-cancer Compound Library clinical trial|Anti-cancer Compound Library cell assay|Anti-cancer Compound Library screening|Anti-cancer Compound Library high throughput|buy Anticancer Compound Library|Anticancer Compound Library ic50|Anticancer Compound Library price|Anticancer Compound Library cost|Anticancer Compound Library solubility dmso|Anticancer Compound Library purchase|Anticancer Compound Library manufacturer|Anticancer Compound Library research buy|Anticancer Compound Library order|Anticancer Compound Library chemical structure|Anticancer Compound Library datasheet|Anticancer Compound Library supplier|Anticancer Compound Library in vitro|Anticancer Compound Library cell line|Anticancer Compound Library concentration|Anticancer Compound Library clinical trial|Anticancer Compound Library cell assay|Anticancer Compound Library screening|Anticancer Compound Library high throughput|Anti-cancer Compound high throughput screening| a time-dependent mode. The ˙OH induction by the complex-H2O2 system was investigated in the conditions of gel electrophoresis

experiments (50 μM concentration of both the complex and H2O2). However, only a slight decrease of the NDMA band was observed. The ability to generate superoxide anion by the complex-H2O2 system was also examined by performing a similar test with another reporter molecule-NBT. Likewise, the investigated system failed to induce this type of radicals. The next experiment was carried out using gel electrophoresis by adding sodium azide (singlet oxygen scavenger) to the

reaction mixture. This Torin 2 procedure did not cause the inhibition of the cleavage reaction either. Taken together, the obtained results suggest that the single- and double-stranded DNA cleavage mediated by complex-H2O2, does not occur by an oxidative mechanism. On the other hand, the same reactions performed without hydrogen peroxide do not result in plasmid degradation (Fig. 6, lanes 4, 10). This led us to propose that most Etomoxir solubility dmso probably the active species is copper-oxene or copper-coordinated hydroxyl radical (Sigman et al., 1991; Baron et al., 1936). The reactive species remain tightly bound to copper(II), thus preventing them from being deactivated by radical Amylase scavengers. A copper-oxene or a resonance hybrid of a

copper(II)-hydroxyl radical species generates a deoxyribose-centered radical by C-1 hydrogen abstraction (Sigman et al., 1991; Baron et al., 1936), and is probably responsible for plasmid DNA cleavage in the studied case. In vitro cytotoxic studies The anticancer activity of MTX, CuCl2, Cu(II)–MTX, and cisplatin against two selected cell lines: mouse colon carcinoma (CT26) and human lung adenocarcinoma (A549) were investigated. The evaluation of the cytotoxic activity of the compounds was carried out by the MTT assay, based on the ability of mitochondrial dehydrogenases in the viable cells to cleave the tetrazolium rings of MTT and to form dark blue membrane-impermeable crystals of formazan. The surviving fraction was determined by the relationship between the optical absorbance of dissolved formazan into a colored solution and the number of viable cell. The IC50 values were derived from dose–response curves and are summarized in Table 3. Cytotoxic study in vitro revealed that Cu(II)–MTX exhibits considerable toxicity toward both tested cell lines. The IC50 values obtained for the complex were in most cases lower than those for MTX and CuCl2. Generally, the greatest effect was observed on both cell lines after 4 h of incubation with the tested samples (Table 3).