155 Serous 75 7 17 17 34   Non serous 21 1 10 4 6   Residual tumor after initial laparotomy           0.000* < 1 cm 42 1 21 10 10   ≥ 1 cm 53 7 6 11 29   Undetermined 1 0 0 0 1   Differentiation           0.199 Well-differentiated 27 4 11 6 6   Moderately-differentiated 23

1 8 3 11   Poorly-differentiated 39 3 7 11 18   Undetermined 7 0 1 1 5   FIGO staging           0.003* I 17 1 10 1 5   II 19 0 10 4 5   III 53 6 7 13 27   IV 7 1 0 3 3   Serum C646 mw CA125           0.301 ≥ 500 52 5 11 13 23   < 500 41 3 16 7 15   Undetermined 3 0 0 1 2   *P < 0.05 EOC = epithelial ovarian cancer; AM = Adrenomedullin; FIGO = International Federation of Gynecology and Obstetrics Follow-up information was available for 82 EOC patients with survival periods ranging from 2 to 89 months (median = 36 months). Survival curves for EOCs were stratified according to AM expression (Figure 2). By using the Kaplan-Meier method, we indicated that both survival time and disease-free time for patients were linked to AM expression

status (disease-free time, P = 0.020, Figure 2A; overall survival time, P = 0.030, Figure 2B). By using univariate Cox proportional Natural Product Library supplier analysis, AM expression was statistically correlated to disease-free survival and overall survival (P < 0.05, Table 2). By using multivariate Cox proportional analysis, considering all the clinical parameters and AM expression, FIGO staging was an independent factor of disease-free survival prognosis prediction, and both age and disease-free time were independent factors predicted EOC over-all survival prognosis Idelalisib mw (P < 0.05, Table 3). Figure 2 Correlation between AM status and EOC patient prognosis. Kaplan-Meier curves for disease-free survival rate of EOC patients according to the AM expression status (A); Kaplan-Meier total survival curves of EOC patients according to the AM expression status (B). Both survival time and disease-free time of patients were linked to AM expression status (Log-rank test, P = 0.020; P = 0.030).

Table 2 Univariate Cox proportional hazards regression analyses of clinicopathological variables and AM expression for EOC patient outcome   Disease-free survival Overall survival Variables           Relative risk (95%CI) P value Relative risk (95%CI) P value Age(≥ 55 years) 1.663(0.985-2.808) 0.057 2.174(1.201-3.935) 0.010* Differentiation 1.542(1.084-2.193) 0.016* 1.449(0.971-2.161) 0.069 FIGO staging(II-IV) 4.883(1.937-12.309) 0.001* 5.285(1.630-17.131) 0.006* Residual tumor after         initial laparotomy (≥ 1 cm) 2.776(1.598-4.824) 0.000* 2.760(1.458-5.227) 0.002* AM expression 1.878(1.081-3.265) 0.025* 2.014(1.052-3.852) 0.035* Disease-free time     0.925(0.904-0.946) 0.000* *P < 0.