05 respectively)(Table 2). Table 2 Association of Lamin A/C immunostaining with clinicopathological parameters in 126 cases of primary GC Clinicopathological variable Cases (n = 126) Lamin A/C p -value     positive (%) negative (%)       n = 70 n = 56   Gender       0.410    male 88 51 (58.0) 37 (42.0)      female 38 19 (50.0) 19 (50.0)   Age (years) a       0.905    < 56 60 33 (55.0) 27 (45.0)      ≥ 56 66 37 (56.1) 29 (43.9)   Tumour size

(cm) a       0.902    < 5 78 43 (55.1) 35 (44.9)      ≥ 5 48 27 (56.3) 21 (43.7)   Depth of invasion       0.870    T1 9 6 (66.7) 3 (33.3)      T2 22 12 (54.5) 10 (45.5)      T3 75 42(56.0) 33 (44.0)      T4 20 10 (50.0) 10 (50.0)   Lymph node metastasis b       0.550    N0 42 23 (54.8) 19 (45.2)      N1 36 22 (61.1) 14 (38.9)      N2 38 18 (47.4) 20(52.6)      N3 10 7(70.0) 3 (30.0)   Distant metastasis       0.659    M0 101 55 (54.5) 40 (45.5)      M1 25 15(60.0) https://www.selleckchem.com/products/GDC-0941.html 10 (40.0)   Staging       0.894    I 17 10 (58.8) 7 (41.2)      II 27 14 (51.9) 13 (48.1)      III 47 25 (53.2) 22 (46.8)      IV 35 21 (60.0) 14 (40.0)   Differentiation       0.034c    well 19 15(78.9) 4 (21.1)      moderate 20

13(65.0) 7 (35.0)      poor 67 35(51.6) 32 (48.4)      undifferentiated 20 7 (35.0) 13 (65.0)   agrouping of age and tumour size was performed according to median. b grouping of staging and selleck kinase inhibitor lymph node metastasis was performed according to UICC classification (TNM 1997). cstatistical 4SC-202 solubility dmso significance Montelukast Sodium (p < 0.05) Figure 4 Immunohistochemical detection of Lamin A/C protein expression in

GC and surrouding non-cancerous tissues. Positive staining was mostly seen on nuclear of epithelial cells. (A) positive staining of Lamin A/C in normal gastric mucosa(× 100). (B) negative staining of Lamin A/C in well-differentiated gastric carcinoma(× 100). (C) negative staining of Lamin A/C in moderately differentiated gastric carcinoma(× 100). (D) negative staining of Lamin A/C in gastric signet-ring cell carcinoma(× 100). T, GC; N, corresponding non-cancerous tissues. The right upper frame of each figure showing high-power field(× 400). Correlation between lamin A/C expression and patients’ survival Using Kaplan-Meier curve method, we evaluated the relationship between the lamin A/C expression and the outcome of 126 patients. The overall survival rates were 58.6% and 44.6%, respectively, in patients with positive and negative lamin A/C expression. Of 70 lamin A/C immunohistochemical positive-staining patients, the median survival time is 45.0 ± 5.5 months, while that of 56 negative-staining patients is 26.0 ± 4.2 months. There was a significantly longer median survival time in the lamin A/C protein-positive group than in the negative group (P = 0.034, log-rank test; Fig. 5).