of 7 oaline, did not evoke an increased incidence of 7 or more leucocytic foci in the hind mammae for only 1 mamma of 48 contained 7 foci 21 days after such inoculations. The prevalence of lesions in mammae 21 days after inoculation of pertussis vaccine combined with Freund,s complete adjuvant emulsified SGX-523 with mammary homogenate did not differ significantly from that found in animals given only Freund,s complete adjuvant and mammary homogenate. TABLE IV. Effect of Pertussis Vaccine on Numbers of Leucocytic Foci in Hind Mammae of Oestrogenized Female Rats at 3 Weeks Inoculum examined 0 1 2 3 4 5 6 7 Pertussis alone 12 4 2 3 3 0 0 0 0 Pertussis FCA saline 36 8 9 7 4 4 2 1 1 Pertussis FCA mamma 51 15 6 7 6 0 5 2 10 Effect of antilymphocyte serum The mean lymphocyte count of the post mortem venous blood of the animals administered antilymphocyte serum was reduced from 18,800 mm 3 to 9280 mm 3 whereas the erythrocyte, neutrophil and monocyte parameters were unchanged.
The intramammary lymph nodes of the antilymphocyte serum treated rats exhibited variable suppression of the paracortical nodules, and expansion and plasmacytosis of the medullary cords together with enhanced germinal centre formation in the cortical follicles. All sera from the rats given antilymphocyte serum produced precipitin arcs Gemcitabine in the agar gel opposite wells containing rabbit serum. The administration of antilymphocyte serum concurrently with the inoculation of lactating mammary homogenate and Freund,s complete adjuvant reduced the proportion of hind mammae containing 7 or more leucocytic foci at 21 days from 21.
4 to 5.5 . Furthermore, the composition of the foci in the TABLE V. Effect of Antilymphocyte Serum on Numbers of Leucocytic Foci in Hind Mammae of Oestrogenized Rats Inoculated with FCA mamma at Base of Tail 3 Weeks Previously No. mammae with specified no. foci No. mammae, A Inoculum examined 0 1 2 3 4 5 6 7 FCA mamma alone 75 7 15 12 9 7 5 4 16 FCA mamma antilymphocyte serum 18 7 3 2 1 3 0 1 1 mammiae of the animals administered antilymphocyte serum differed strikingly from that seen after inoculation of mammary homogenate and Freund,s complete adjuvant alone. The mononuclear component of the exudate was suppressed and replaced by eosinophil and neutrophil granulocytes. The character and prevalence of the leucocytic foci in the hind mammae after inoculation of an emulsion of mammary homogenate and Freund,s complete adjuvant differed from those seen in control animals administered oestrone alone.
Advantage was taken in these experiments of the sparse leucocytic infiltration in the early stages of mammary response to oestrogens, for longer periods of oestrogenization are associated with pronounced spontaneous inflammatory infiltration in the mammae of mice and rats. The sites at which the intradermal inoculations were made did not have significant effect upon the numbers of leucocytic foci observed 21 days afterwards. This was confirmed by the similarity of the mammary reactions in thoracic and abdominal regions after inocula at the base of the tail. The only differences in the mammae that did appear related to the site of inoculation were perilymphangitic lesions. These were, however, excluded bv definition in the assessment of the intramammary foci. Of the various inoculati