A single group received an antioxidant rich diet program when a further group received 3 kiwifruits every day. The antioxidant rich diet program food products have been included in exchange for foods in their habitual diet plan. The meals exchange was discussed with the study nutritionist, but the participants had been free of charge to choose which foods to replace. The kiwifruits were added for the habitual diet. The handle subjects continued their habitual diet regime with a few restrictions. In total, one hundred participants completed the study. Blood samples were collected at Oslo University Hospital, Ullev l, Norway. Ten indivi duals from every group had been randomly selected for gene expression analysis of entire blood RNA applying Affyme trix Human Genome U133 Plus two. 0 arrays. All statistics on baseline traits and plasma biomarkers within this paper are provided for this subgroup only.
The subjects in both intervention groups received meals products weekly. Inside the antioxidant group these selleck chemicals Palbociclib included green tea, dog rose juice, cranberry juice, aronia juice, unsweetened bilberry juice, bilberry jam, bilberries, blackberries, strawberries, raspberries, pomegranate, dark blue grapes, Brussels sprouts, broccoli, red cabbage, kale, blue potatoes, tomatoes, dark chocolate, bread with pecan nuts and sunflower seeds, walnuts, olive oil, rosemary, thyme and oregano. The kiwifruit group received a bag of 21 kiwifruits. Weekly amounts with the food products supplied for the antioxidant rich eating plan group are listed in Table 1. The foods that had been not consumed have been registered weekly within a custom created questionnaire. Subjects inside the control group had been monitored at stick to ups every single second week.
For complete blood genome analy sis, samples had been collected utilizing PAXgene Blood RNA Tubes in the time of rando mization and in the finish of selleck chemical the intervention period. All investigators performing sample analyses or statistics had been blinded until statistical analyses were performed. The study was authorized by the regional ethics commit tee for healthcare study, and all participants gave their written, informed consent. The study is regis tered at. gov with Identifier NCT00520819. The trial registry name is Oslo Antioxi dant Study. Comparison of baseline traits and dietary intake Kruskal Wallis 1 way evaluation of variance was utilized to evaluate regardless of whether baseline values or adjustments through the intervention period differed in between the groups. Where considerable outcomes had been obtained making use of Kruskal Wallis, a Mann Whitney nonparametric test was performed to examine the median values among the groups. Alterations through the intervention period had been calculated by subtracting the baseline value in the postinterven tion worth. All statistics had been performed working with SPSS ver sion 14.