The patient’s past history revealed a 10-year history of psoriasis and chronic obstructive pulmonary disease treated with methotrexate and prednisolone. Pathology was assessed by histology
and molecular biologic analyses.\n\nA repeated bone marrow biopsy revealed multiple intracellular particles which were identified as Leishmania amastigotes. Indirect immunofluorescence AZD8186 inhibitor as well as enzyme-linked immunosorbent assay (ELISA) of patient’s serum showed specific anti-Leishmania antibodies. Despite rapid initiation of systemic therapy, the patient died of a secondary infection. Post mortem, PCR and sequencing revealed synchronous infection with Leishmania donovani/infantum complex and Leishmania major.\n\nDiagnosis of patients with complex clinical features is challenging even for experienced clinicians. Critical interpretation of findings and, if necessary, repetition of invasive examinations may be necessary for proper diagnosis. Increasing numbers of immunocompromised patients (iatrogenic, HIV) will expand the spectrum of rare infectious
diseases including visceral leishmaniasis.”
“Theileria pony, the most important bovine theilerial species in sub-Saharan Africa, causes widespread mortality and morbidity in endemic areas. A survey was conducted using buffy-coat specimens from 60 apparently healthy adult communally herded Nguni-type cattle at the northeastern edge of the Hluhluwe-iMfolozi Park selleck chemicals to determine, by means of PCR and Reverse Line Blot (RLB) hybridisation, the occurrence of Theileria and Babesia species. The presence of Trypanosoma species was determined using PCR-RFLP Results showed that 6.7 % of the specimens were positive for Theileria
parva. This significant finding suggests that cattle in South Africa, and not only African buffaloes (Syncerus caffer), may be subclinical carriers of T parva. Other species identified were T mutans find more (83.3 %), T. velifera (70.0 %), Theileria sp. (sable) (46.8 %) and T. taurotragi (1.7 %). Two specimens (3.3 %) were positive for Babesia bovis and single specimens (1.7 %) positive for B. bigemina and B. rossi, respectively. Mixed infections, of up to 4 species, were common (65.0 %). Only 1 specimen was found to be positive for Trypanosoma lynx, and 2 for T. theileri, of which only the first species is pathogenic.”
“Pseudomonas aeruginosa is an opportunistic pathogen of plants and animals, which produces virulence factors in order to infect or colonize its eukaryotic hosts. Cyclodipeptides (CDPs) produced by P. aeruginosa exhibit cytotoxic properties toward human tumor cells. In this study, we evaluated the effect of a CDP mix, comprised of cyclo(L-Pro-L-Tyr), cyclo(L-Pro-L-Val), and cyclo(L-Pro-L-Phe) that were isolated from P. aeruginosa, on two human cancer cell lines.