Results and discussion Fabrication of nanopore-based device In our experiment, PC ultrafiltration membranes are employed as nanopore arrays, whose size and distribution are characterized using an atomic force microscope. The AFM image shown in Figure 2 gives the size and distribution information of the nanopore arrays: their pore size is 50 nm or so, and they are distributed randomly in the membrane. The micropores in the Si3N4 films were fabricated using focused Ga+ Fosbretabulin research buy beam. Obviously, the size and shape of the pore are mainly determined by the energy of the Ga+ beam and irradiation time. Generally speaking, greater beam energy corresponds
to rather faster processing speed. Meanwhile, the irradiation this website time should exceed a threshold value to guarantee the film being penetrated. In a certain range, the pore size will gradually increase with increasing irradiation time. By controlling the proper beam energy and irradiation time, four Si3N4 pores with sizes of 0.47, 0.88, 1.5, and 2.0 μm are obtained, as shown in Figure 3. If these pores are regarded as ideal round, the calculated pore areas are 0.16, 0.61, 1.77, and 3.14 μm2, respectively. Considering the calculated pore areas and the distribution status of the nanopore, theoretical amounts of ‘uncovered’ nanopores
are 0.96, 3.66, 9.84, and 18.84, respectively. At the same time, the total amounts of the uncovered nanopores are also influenced by the heterogeneity of their distribution and other related to factors (for example, it is difficult to control PDMS to exactly arrive at the edge of the micropore. Less mobility of PDMS at the beginning of the solidification may make it exceed the edge of the micropore, which will result in the decrease of effective pore size or even pore closing). According to our experimental experience, if the size of
Si3N4 pore is less than 1 μm, it is difficult to guarantee the success of further ionic current detection. In our experiment, micropores with sizes of 1.5 and 2.0 μm have been employed. Figure 3 SEM images of the Si 3 N 4 micropores with different diameters in Si-Si 3 N 4 hybrid structures. (a) 0.47 μm, (b) 0.88 μm, (c) 1.5 μm and (d) 2.0 μm. Ionic currents induced by biomolecule translocation The sensing device based on PC membranes {Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|buy Anti-diabetic Compound Library|Anti-diabetic Compound Library ic50|Anti-diabetic Compound Library price|Anti-diabetic Compound Library cost|Anti-diabetic Compound Library solubility dmso|Anti-diabetic Compound Library purchase|Anti-diabetic Compound Library manufacturer|Anti-diabetic Compound Library research buy|Anti-diabetic Compound Library order|Anti-diabetic Compound Library mouse|Anti-diabetic Compound Library chemical structure|Anti-diabetic Compound Library mw|Anti-diabetic Compound Library molecular weight|Anti-diabetic Compound Library datasheet|Anti-diabetic Compound Library supplier|Anti-diabetic Compound Library in vitro|Anti-diabetic Compound Library cell line|Anti-diabetic Compound Library concentration|Anti-diabetic Compound Library nmr|Anti-diabetic Compound Library in vivo|Anti-diabetic Compound Library clinical trial|Anti-diabetic Compound Library cell assay|Anti-diabetic Compound Library screening|Anti-diabetic Compound Library high throughput|buy Antidiabetic Compound Library|Antidiabetic Compound Library ic50|Antidiabetic Compound Library price|Antidiabetic Compound Library cost|Antidiabetic Compound Library solubility dmso|Antidiabetic Compound Library purchase|Antidiabetic Compound Library manufacturer|Antidiabetic Compound Library research buy|Antidiabetic Compound Library order|Antidiabetic Compound Library chemical structure|Antidiabetic Compound Library datasheet|Antidiabetic Compound Library supplier|Antidiabetic Compound Library in vitro|Antidiabetic Compound Library cell line|Antidiabetic Compound Library concentration|Antidiabetic Compound Library clinical trial|Antidiabetic Compound Library cell assay|Antidiabetic Compound Library screening|Antidiabetic Compound Library high throughput|Anti-diabetic Compound high throughput screening| containing nanopore arrays was used to detect the ionic currents modulated by the biomolecule’s translocation. KCl solutions of 0.001, 0.01, and 0.1 mol/L were employed as electrolytes, and IgG was used as analyte. As mentioned above, there are many, many nanopores in the PC nanopore membrane (pore density six pores per μm2). If only the PC nanopore membrane is used, the effective nanopore number is about 106 to 107, which is a very big amount. From a probabilistic perspective, a lot of IgG molecules will pass through the nanopore arrays simultaneously.