Enteritidis (wt) and ΔSPI2 mutant. n.i. – non-infected mice. * – t-test different from the non-infected mice at P < 0.05. Finally we tested whether the depletion of NK cells could be caused by their migration to the caecal lamina propria. We therefore infected mice with wild type S. Enteritidis and ΔSPI2 mutant, and besides the spleen we also determined the counts of the NK cells in blood and ZVADFMK the lamina propria. In blood, a significant decrease in NK cells post
wild-type S. Enteritidis infection was observed. In the lamina propria, the numerical increase in NK cells was observed although this increase did not reach statistical significance (Figure 8). Figure 8 Distribution of NK cells in spleen, blood and caecal lamina propria of mice infected with the wild type
S . Enteritidis (wt) and ΔSPI2 mutant as determined in the animal infection 4. n.i. – non-infected mice. * – t-test different from the non-infected mice at P < 0.01. Discussion Similar to the observations of others, progress of the infection in mice, characterised by fecal shedding, fatalities, liver and spleen colonisation and liver injury, was dependent on the presence of SPI-2 but not any other SPI [3, 17, 18]. The exclusivity of SPI-2 dependence for S. Enteritidis virulence for mice was such that even in the absence of all remaining SPIs, i.e. in the case of SPI2o mutant, this mutant was capable of causing typhoid similar to that caused by the wild-type
strain. This observation ICG-001 purchase was slightly unexpected for the mutants without SPI-1. However Murray and Lee already reported on minimal influence of the removal of the whole SPI-1 on the virulence of S. Typhimurium for Balb/C mice  and also single gene mutants in sopB, sopD or sipA were only weakly attenuated [19, 20] or the attenuation Casein kinase 1 was expressed only as a minor delay in mean time to death . In addition, dose dependent difference in the virulence of sopB mutant of S. Typhimurium was described  and since we used only a single dose corresponding to 100× LD50, minor phenotypic differences associated with the presence or absence of SPI-1 could remain undetected. The infection did not influence the counts of T- and B-lymphocytes in the spleen at the time of sampling, similarly to the findings of Geddes et al . We did not even observe an increase in γδ T-lymphocytes although these were reported to increase in mice after infection with a virulence plasmid-cured derivative of S. Choleraesuis . Although there were no changes in these cell populations, general immunosuppression has been observed when PHA was used as the mitogen for stimulation. Since the immunosuppression was not observed when ConA and PHW were used for the stimulation, it can be expected that the population which was primarily immunosuppressed was that represented by the CD4 Th lymphocytes .