his study exhibits previousy reported ctivitiesMarch,, incuding hemggutinting, ribosome inctivting,Marchgctosespeciﬁc ctivities. Bioinformtic studies reve cose retionship between MCMarchthe cssic type II RIP, ricin Suppementry S. though very toxic, ricinMarchits engineered products hve been reported with potent ntitumor ctivity chrcterized by Seliciclib speciﬁcity, high efﬁccy,Marchstbiity . The resuts of the present study iicte tht MC exhibited seective cytotoxicity on representtive types of NPC March by the regution of MPKsMarchcspse cscde. The cytotoxicityMarchntiproifertive ctivity of MC on NPC March were higher thn those in the norm NP MarchMarchThis my be cused by the numeric difference of moecues recognizbe by MC ectin chin on the surfce of the March, for some investigtions fou tht chnges in ce surfce sugrs re ssocited with the deveopment of cncerMarchSubsequent studies showed tht iuction of tumor ce poptosis ws responsibe for the MC effect.
MC incresed the proportion of poptotic March, cused chromtin coenstion,Marchnucer frgmenttionMarchwhich re unique morphoogic nucer chnges of poptosis . the sme time, G phse cecyce rrestMarchmitochori dmge were detected. It seems tht MCiuced mitochori dmge seems to be the cuse rther thn the effect of MCiuced ce deth becuse the zafirlukast phenomenon ws detectbe s ery s hours fter tret ment dt not shown. These resuts gree with the pre viing response of most cncer March exposed to chemo therpeutic ponents March. We then tried to reve the moecur bsis invoved in MCiuced G cecyce rrest. MC cused G ce cyce rrest which ws prtiy contributed by decresed eves of cycin DMarchphosphoRb, which re key members invoved in ce proifertionMarchG cecyce progression . The resuts re mensurte with those brought bout by the dministrtion of other chemotherpeutic regents on NPC March, such s Grifoin . Furthermore, previous reports iicte tht the G cecyce rrest is reguted in p,Marchpdepeent mnner March Cncer Prev Res; Jnury Cncer Prevention Reserch Downoded from cncerpreventionreserch.
crjourns on March , mericn ssocition for Cncer Reserch Pubished OnineFirst September March CPR The ntitumor ctivity of Momordic Chrnti ectin on NP or b piepeent trnscription iuction of p , or c piepeent mnner . Prdox icy, we fou tht MC perturbs G signing dist to Phlorizin Phloridzin|Phlorizin inhibitor pMarchp expression. Western bot nysis iicted tht MC doseMarchtime depeenty decresed the expres sion of both proteins in CNEMarchCNE March dt not shown. These dt re in keeping with resuts from other workers using the sme ce ines March This is expin be becuse mounting evidence iictes tht p mut tion is mong the most mon genetic events in the deveopment of humn cncer ,Marchboth types of NPC March hve identic G rginine to C eonine chnges t codonMarchof p . Moecur inkges between MPKsMarchvriety of ceur progrms such s proifertion, differentition, deveopment, trnsformtion,Marchpoptosis hve mde the signing cscde n object of intense reserch in recent yers .
The ctivtion of p MPKMarchJNK hs been reported to py signiﬁcnt function roe in ce deth iuction .Marchcuriousy, ERK phosphorytion hs been inked with both ntitumorMarch protumorMarchctivities. Our resuts show tht MC iuced the phos phorytion of p MPK in both NPC tumor MarchMarchso incresed the ctivtion of JNK from different time points. It is importnt to note tht MC cused n increse of pERK in CNE March, s we s ttenuted ERK phosphorytion in CNE MarchMarchWe further fou tht MC coud iuce NO production in NO synthsedepeent wy B. Phrmcoogic interruption of p MKP sign ing, by the speciﬁc inhibitor , wekened MC ethityMarchthe downstrem NO production, iicting tht this pthwy pys n importnt prt in ce deth iuction. The NOiucing ctivity of MC is of more thn trivi interest becuse unphysioogicy high eves of extrceur NO cn iuce poptosis or necrosis . Both type IMarchtype II RIPs,Marchectins , hve been shown with NOiucing ctivity in mouse Medical mcrophges or tumor March,Marchwhich chins of MC contributes to the production of NO wits eucidtion. poptosis is executed by cspsesMarchcspse is key protese ssocited with DN frgmenttion March poptosis.